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] "en" => array:19 [ "idiomaDefecto" => true "titulo" => "Analysis of antioxidant activity on cocktail honey products as female pre-conception supplements" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "S202" "paginaFinal" => "S205" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Riska Reviana, Andi Nilawati Usman, Indah Raya, Aliyah, Andi Dirpan, Aryadi Arsyad, Fendi Fendi" "autores" => array:7 [ 0 => array:3 [ "nombre" => "Riska" "apellidos" => "Reviana" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 1 => array:4 [ "nombre" => "Andi Nilawati" "apellidos" => "Usman" "email" => array:3 [ 0 => "andinilawati@pasca.unhas.ac.id" 1 => "nilawatiandi@gmail.com" 2 => "pmc@agri.unhas.ac.id" ] "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 2 => array:3 [ "nombre" => "Indah" "apellidos" => "Raya" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 3 => array:2 [ "apellidos" => "Aliyah" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 4 => array:3 [ "nombre" => "Andi" "apellidos" => "Dirpan" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 5 => array:3 [ "nombre" => "Aryadi" "apellidos" => "Arsyad" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 6 => array:3 [ "nombre" => "Fendi" "apellidos" => "Fendi" "referencia" => array:5 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 3 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] 4 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] ] "afiliaciones" => array:5 [ 0 => array:3 [ "entidad" => "Midwifery Study Program, Graduate School, Hasanuddin University, Indonesia" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Department of Chemistry, Faculty of Science, Hasanuddin University, Makassar, Indonesia" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Hasanuddin University, Makassar, Indonesia" "etiqueta" => "c" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Department of Agricultural Technology, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia" "etiqueta" => "d" "identificador" => "aff0020" ] 4 => array:3 [ "entidad" => "Research and Community Service, Wuna Agricultural Science University, Indonesia" "etiqueta" => "e" "identificador" => "aff0025" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0010" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 635 "Ancho" => 1341 "Tamanyo" => 62690 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">The curve of <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Cocktail honey is a mixture of royal jelly, bee bread, and honey through mixing and laboratory test results.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a> Royal jelly is a bee product other than honey which is produced from the hypopharyngeal glands of young bees containing polyphenols, several enzymes such as glucose oxidase, and superoxide dismutase, vitamins B1, B2, B3, and vitamin C, which act as antioxidants.<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">2</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Honey contains organic acids, amino acids, vitamins A, B complex, C, D, E, and K, electrolytes, elements such as copper, zinc, minerals with a pH between 3 and 4, enzymes, glucose, and fructose.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">3</span></a> Honey also plays an active role as an antioxidant.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">3</span></a> Honey also plays an active role as an antioxidant. With ingredients such as flavonoids, phenolic acids, enzymes, and vitamins.<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">4</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">The Trigona genus bees produce trigona type honey.<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">5</span></a> A study conducted by Nilawati et al. (2016) stated that trigona honey contains a high total phenolic amounting to 106<span class="elsevierStyleHsp" style=""></span>mg/100<span class="elsevierStyleHsp" style=""></span>g; vitamin E at 9.95<span class="elsevierStyleHsp" style=""></span>μg/g; vitamin C 302.85<span class="elsevierStyleHsp" style=""></span>μg/g; and quercetin of 58.8%.<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">6</span></a> Trigono honey is a type of honey that comes from the forest, contains antioxidants that are high in flavonoids, vitamins, phenolic acids, and polyphenols.<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">7</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">According to Oddo et al. (2008), research shows that the examination of the antioxidant activity of trigona honey using DPPH of 48.03<span class="elsevierStyleHsp" style=""></span>ppm included in the category of strong antioxidants and flavonoid levels of 10.52<span class="elsevierStyleHsp" style=""></span>mg.<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">8</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Bee bread is also a bee product from the fermentation of a mixture of pollen, nectar, and addition of bee saliva, which is inoculated by various bacteria and yeast, contains protein, lactic acid, which is a preservative,<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">9</span></a> vitamins (C, B, K, P and E), minerals 3%, carbohydrates 24–35%, carotenoids, and polyphenols such as anthocyanins and flavonoids.<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">10</span></a> As well as other active components such as the enzymes saccharase, amylase, phosphatase, a hormone that contains antioxidants.<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">11</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">Various antioxidants have been found in plants with many phytochemicals with various bioactivities, including polyphenols, carotenoids, tocopherol, and ascorbic acid.<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">12</span></a> Bee products contain antioxidants such as royal jelly, bee bread, and honey. The antioxidant activity of honey has been praised in previous studies and found that honey can suppress oxidation activity up to 50%, which is worth in the DPPH test 7.5–109<span class="elsevierStyleHsp" style=""></span>mg/ml.<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">13</span></a> Besides, other studies mention that bee bread contains phenolic compounds and flavonoids that play a role in antioxidant activity.<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">14</span></a></p><p id="par0035" class="elsevierStylePara elsevierViewall">This is in line with Rahma et al. (2014) research, stating that there are antioxidants in dorsata honey and trigona honey. The antioxidant action in honey can reduce or inhibit free radicals so that cell damage does not increase.<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">15</span></a></p><p id="par0040" class="elsevierStylePara elsevierViewall">This study was conducted to determine the antioxidant activity found in cocktail honey using the DPPH test method (2,2-diphenyl-1-picrylhydrazyl) using UV–vis spectrophotometry to know the value of free radical scavenging activity expressed by IC<span class="elsevierStyleInf">50</span> values (inhibitory concentration). It is expected to be one of the alternative therapies in treating oxidative stress in the body, especially in the reproductive problems of pre-conception women.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Methods</span><p id="par0045" class="elsevierStylePara elsevierViewall">Test research from the honey cocktail sample, namely a mixture of 100<span class="elsevierStyleHsp" style=""></span>g of royal jelly, 100<span class="elsevierStyleHsp" style=""></span>g of bee bread, and 100<span class="elsevierStyleHsp" style=""></span>g of Trigona honey, to see the antioxidant activity found in cocktail honey with the DPPH test.</p><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Time and place of research</span><p id="par0050" class="elsevierStylePara elsevierViewall">The study was conducted in June 2020 in Makassar, South Sulawesi. Testing samples were done at two places, Mathematics and Natural Sciences Laboratory of the Faculty of Mathematics and Natural Sciences, Hasanuddin University, and the Biochemical Laboratory of Mathematics and Natural Sciences, Hasanuddin University, Makassar.</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Tools and materials</span><p id="par0055" class="elsevierStylePara elsevierViewall">The tools used in this study are glassware (Pyrex), stopwatches, analytical scales, measuring flasks, pipettes, UV–vis spectrophotometers (Shimadzu type 2450). Materials used in this study include cocktail honey made from a mixture of 30<span class="elsevierStyleHsp" style=""></span>ml honey:30<span class="elsevierStyleHsp" style=""></span>ml bee bread:30<span class="elsevierStyleHsp" style=""></span>ml royal jelly, a solution of methanol, distilled water, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) pa (Sigma-Aldrich material code No. D9132-1G).</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Research design</span><p id="par0060" class="elsevierStylePara elsevierViewall">The making of cocktail honey is from a mixture of 30<span class="elsevierStyleHsp" style=""></span>ml honey:30<span class="elsevierStyleHsp" style=""></span>ml bee bread:30<span class="elsevierStyleHsp" style=""></span>ml royal jelly. First, the prepared test solution is put into a test tube. Then, each tube was added with 1<span class="elsevierStyleHsp" style=""></span>ml of 0.4<span class="elsevierStyleHsp" style=""></span>mM DPPH solution, added 4<span class="elsevierStyleHsp" style=""></span>ml of methanol to 5<span class="elsevierStyleHsp" style=""></span>ml, and homogenized. The blank, test and comparative solutions were immediately incubated for 30<span class="elsevierStyleHsp" style=""></span>min in a dark room of light. At the maximum wavelength of the Biochemical Laboratory of the Faculty of Mathematics and Natural Sciences, Hasanuddin University, the readings were 515<span class="elsevierStyleHsp" style=""></span>nm using a UV–vis spectrophotometer. The uptake obtained was then recorded and calculated as a percentage of free radical activity inhibition.<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">16</span></a> Thus, the value of the free radical damping activity will be known as stated by the IC<span class="elsevierStyleInf">50</span> (inhibitory concentration).</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Research stages</span><p id="par0065" class="elsevierStylePara elsevierViewall">The raw material for making cocktail honey products is 30<span class="elsevierStyleHsp" style=""></span>ml honey:30<span class="elsevierStyleHsp" style=""></span>ml bee bread:30<span class="elsevierStyleHsp" style=""></span>ml royal jelly obtained from the Faculty of Forestry, Hasanuddin University. The material is homogeneous until it merges. Cocktail honey products were tested for DPPH to determine the antioxidant activity by weighing 5<span class="elsevierStyleHsp" style=""></span>mg DPPH dissolved with 20<span class="elsevierStyleHsp" style=""></span>ml of absolute methanol in a flask.</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Research parameters</span><p id="par0070" class="elsevierStylePara elsevierViewall">DPPH test (2,2-diphenyl-1-picrylhydrazyl) using UV–vis spectrophotometry due to finding out the antioxidant activity.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">3</span></a> A compound can be said to have antioxidant activity if the compound can donate its hydrogen atom to bind to DPPH to form a reduced DPPH characterized by looking at the change in color of each sample after incubation with DPPH. Increasingly the loss of purple or yellowing color.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a> The determination of antioxidant activity is expressed in IC<span class="elsevierStyleInf">50</span> (μg/ml) as antioxidant capacity. The IC<span class="elsevierStyleInf">50</span> value is defined as the concentration of test compounds that can inhibit free radicals by as much as 50%. The smaller IC<span class="elsevierStyleInf">50</span> value, the higher the free radical reduction activity.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">3</span></a> The IC<span class="elsevierStyleInf">50</span> value category is powerful if the IC<span class="elsevierStyleInf">50</span> value <10<span class="elsevierStyleHsp" style=""></span>μg/ml, strong if the IC<span class="elsevierStyleInf">50</span> value is between 10 and 50<span class="elsevierStyleHsp" style=""></span>μg/ml, mild if the IC<span class="elsevierStyleInf">50</span> value is between 50 and 100<span class="elsevierStyleHsp" style=""></span>μg/ml, weak if the IC<span class="elsevierStyleInf">50</span> value is between 100 and 250<span class="elsevierStyleHsp" style=""></span>μg/ml and not active if IC<span class="elsevierStyleInf">50</span> is above 250<span class="elsevierStyleHsp" style=""></span>μg/ml.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a></p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Data processing</span><p id="par0075" class="elsevierStylePara elsevierViewall">Data cannot be processed using statistical analysis because there is too little amount of data presented. The data is calculated using a formula to find the percentage value of the antioxidant activity, as well as the value of X or IC<span class="elsevierStyleInf">50</span> which is calculated using the line equation obtained from the % value of the antioxidant activity and the concentration value plotted on a graph, where the concentration value is on the <span class="elsevierStyleItalic">X</span>-axis and the % activity is on the <span class="elsevierStyleItalic">Y</span>-axis.</p><p id="par0080" class="elsevierStylePara elsevierViewall">The concentration value comes from the value of the preliminary test on the sample carried out to find out one point or point desired or achieved. Absorbance value (<span class="elsevierStyleItalic">A</span>) with a maximum wavelength (<span class="elsevierStyleItalic">λ</span>max) of 515<span class="elsevierStyleHsp" style=""></span>nm was obtained by testing using a spectrophotometer.</p><p id="par0085" class="elsevierStylePara elsevierViewall">The effective concentration value is a number that shows the extract concentration (microgram/milliliter), which can inhibit 50% oxidation. The calculation of the effective concentration value or IC<span class="elsevierStyleInf">50</span> uses the following formula:<elsevierMultimedia ident="eq0005"></elsevierMultimedia></p><p id="par0090" class="elsevierStylePara elsevierViewall">Information: Ac<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>absorbance value of control, A<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>the absorbance value of the sample.</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Results</span><p id="par0095" class="elsevierStylePara elsevierViewall">Antioxidants are valuable compounds to overcome oxidative damage due to free radicals in the body to prevent various diseases.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a> Although some research also states that flavonoid compounds can protect lipids from the oxidation of cell membranes, these compounds play a role in antioxidant activities that are beneficial to human health.<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">17</span></a></p><p id="par0100" class="elsevierStylePara elsevierViewall">In <a class="elsevierStyleCrossRefs" href="#tbl0005">Tables 1–3</a> that have been plotted, the equation of the line is used to find the effective concentration of the honey cocktail to soak the DPPH free radicals or the IC<span class="elsevierStyleInf">50</span> value as shown in the following <a class="elsevierStyleCrossRefs" href="#fig0005">Figs. 1–3</a>.</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0105" class="elsevierStylePara elsevierViewall">Based on <a class="elsevierStyleCrossRef" href="#tbl0020">Table 4</a>, the IC<span class="elsevierStyleInf">50</span> value is the effective concentration of the extract needed to immerse 50% of the activity of the total DPPH, so that the IC<span class="elsevierStyleInf">50</span> value is substituted for the value. After substituting the value of 50 for the <span class="elsevierStyleItalic">y</span> value, the <span class="elsevierStyleItalic">x</span> value is obtained as the IC<span class="elsevierStyleInf">50</span> value. Based on the linear regression equation <span class="elsevierStyleItalic">y</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">bx</span><span class="elsevierStyleHsp" style=""></span>+<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">a</span>, the IC<span class="elsevierStyleInf">50</span> value for the honey cocktail product is obtained at simplo 4220.5041<span class="elsevierStyleHsp" style=""></span>μg/ml, duplo of 4710.0636<span class="elsevierStyleHsp" style=""></span>μg/ml, and triplo of 4802.7263<span class="elsevierStyleHsp" style=""></span>μg/ml.</p><elsevierMultimedia ident="tbl0020"></elsevierMultimedia></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Discussion</span><p id="par0110" class="elsevierStylePara elsevierViewall">The IC50 simplo, duplo, and triplo values show that the IC<span class="elsevierStyleInf">50</span> value is >250<span class="elsevierStyleHsp" style=""></span>μg/ml so that the honey cocktail contains very weak antioxidants (IC<span class="elsevierStyleInf">50</span> value >250). According to the parameters, The IC<span class="elsevierStyleInf">50</span> value category is very strong if the IC<span class="elsevierStyleInf">50</span> value <10<span class="elsevierStyleHsp" style=""></span>μg/ml, strong if the IC<span class="elsevierStyleInf">50</span> value is between 10 and 50<span class="elsevierStyleHsp" style=""></span>μg/ml, mild if the IC<span class="elsevierStyleInf">50</span> value is between 50 and 100<span class="elsevierStyleHsp" style=""></span>μg/ml, weak if the IC<span class="elsevierStyleInf">50</span> value is between 100 and 250<span class="elsevierStyleHsp" style=""></span>μg/ml and not active if IC<span class="elsevierStyleInf">50</span> is above 250<span class="elsevierStyleHsp" style=""></span>μg/ml.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a></p><p id="par0115" class="elsevierStylePara elsevierViewall">The study conducted by Handayani (2018) stated that methanol extract gave positive results on tannins, flavonoids, saponins, alkaloids, and steroids with DPPH test IC<span class="elsevierStyleInf">50</span> values of 683.153<span class="elsevierStyleHsp" style=""></span>μg/ml. At the same time, DCM extract gave positive results of steroids, alkaloids, and tannins with IC<span class="elsevierStyleInf">50</span> values of 701.743<span class="elsevierStyleHsp" style=""></span>μg/ml. Thus, positive n-hexane extract containing alkaloids and tannins IC<span class="elsevierStyleInf">50</span> values 1709.536<span class="elsevierStyleHsp" style=""></span>μg/ml, alkaloid positive water extracts, steroids, tannins, and saponins with IC<span class="elsevierStyleInf">50</span> values of 1698.345<span class="elsevierStyleHsp" style=""></span>μg/ml. Positive honey samples contain all aspects tested with an IC<span class="elsevierStyleInf">50</span> value of 2826.471<span class="elsevierStyleHsp" style=""></span>μg/ml, so it can be concluded that honey samples and each extract have a very weak antioxidant ability.<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">7</span></a></p><p id="par0120" class="elsevierStylePara elsevierViewall">Several factors can influence antioxidant activity, namely the differences in the types of honey-producing bees, the geographical conditions of the plant sources used by the bees.<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">18</span></a> Flavonoids are one component of phenolic compounds which are natural antioxidants derived from plants. Plants that live in the grazing location will affect the content of flavonoids, which are natural antioxidants in the product.<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">19</span></a></p><p id="par0125" class="elsevierStylePara elsevierViewall">The phenolic content also depends on the location and geographic location in beekeeping, which has different geographies and different available plant sources so that the phenolic content also varies.<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">20</span></a> The grazing area with a few plant species will affect the amount of flavonoid content in the product material because the limited number of plants in the grazing area can make it difficult for the bees to search for food due to the different bees flying range. For example, the genus <span class="elsevierStyleItalic">Trigona</span> sp. has a flying range of only 500<span class="elsevierStyleHsp" style=""></span>m radii so that the bees incentivize in the hive area.<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">19</span></a></p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Conclusion</span><p id="par0130" class="elsevierStylePara elsevierViewall">The results showed that the content contained in the honey cocktail contains weak bioactive content by assessing the antioxidant content using DPPH. The phenolic content can cause this also depends on the location, geographic location of grazing, which has different geographical and different available plant sources so that the phenolic content also varies.<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">20</span></a></p><p id="par0135" class="elsevierStylePara elsevierViewall">The difference in the results of antioxidant activity tests using DPPH is caused by the test method and the conditions used in processing, homogeneous ingredients, solvent volume, extraction time, temperature, and pressure in product management. The extraction method process can also influence antioxidant activity, and the conditions used when making products.<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">21</span></a></p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Funding</span><p id="par0140" class="elsevierStylePara elsevierViewall">The author received no financial support for the research, authorship, and publication of this article.</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Conflict of interest</span><p id="par0145" class="elsevierStylePara elsevierViewall">The authors declare no conflict of interest.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:11 [ 0 => array:3 [ "identificador" => "xres1632459" "titulo" => "Abstract" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Objective" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Method" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusion" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec1456611" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 3 => array:3 [ "identificador" => "sec0010" "titulo" => "Methods" "secciones" => array:6 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Time and place of research" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Tools and materials" ] 2 => array:2 [ "identificador" => "sec0025" "titulo" => "Research design" ] 3 => array:2 [ "identificador" => "sec0030" "titulo" => "Research stages" ] 4 => array:2 [ "identificador" => "sec0035" "titulo" => "Research parameters" ] 5 => array:2 [ "identificador" => "sec0040" "titulo" => "Data processing" ] ] ] 4 => array:2 [ "identificador" => "sec0045" "titulo" => "Results" ] 5 => array:2 [ "identificador" => "sec0050" "titulo" => "Discussion" ] 6 => array:2 [ "identificador" => "sec0055" "titulo" => "Conclusion" ] 7 => array:2 [ "identificador" => "sec0060" "titulo" => "Funding" ] 8 => array:2 [ "identificador" => "sec0065" "titulo" => "Conflict of interest" ] 9 => array:2 [ "identificador" => "xack576322" "titulo" => "Acknowledgments" ] 10 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2021-06-28" "fechaAceptado" => "2021-07-30" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec1456611" "palabras" => array:5 [ 0 => "Cocktail honey" 1 => "Antioxidant" 2 => "Nutrition" 3 => "Pre-conception supplement" 4 => "Reproductive health" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Objective</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Cocktail honey is derived from a mixture of honey (<span class="elsevierStyleItalic">trigona</span> sp.), bee bread, and homogeneous royal jelly. The material has a phenolic content rich in antioxidants that are beneficial for women's reproductive health, especially for pre-conception, because it can suppress the content of free radicals in the body. Antioxidants are useful to overcome oxidative damage due to free radicals in the body that prevent various diseases from increasing fertility during pre-conception.</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Method</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">This study used the DPPH (2,2-diphenyl-1-picrylhydrazyl) test method using UV–vis spectrophotometry to express the value of free radical reduction activity as IC<span class="elsevierStyleInf">50</span> (inhibitory concentration) values.</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The DPPH test on cocktail honey products obtained an average yield of 4577.7<span class="elsevierStyleHsp" style=""></span>μg/mL, which was included in the product category was very weak in the antioxidant activity content.</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusion</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">The content contained in the honey cocktail contains weak bioactive content by assessing the antioxidant content using DPPH. The difference in the results of antioxidant activity tests using DPPH is caused by the test method and the conditions used in processing, homogeneous ingredients, solvent volume, extraction time, temperature, and pressure in product management.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Objective" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Method" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusion" ] ] ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Peer-review under responsibility of the scientific committee of the 3rd International Nursing, Health Science Students & Health Care Professionals Conference. Full-text and the content of it is under responsibility of authors of the article.</p>" ] ] "multimedia" => array:8 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 590 "Ancho" => 1341 "Tamanyo" => 58991 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">The curve of <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>.</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 635 "Ancho" => 1341 "Tamanyo" => 62690 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">The curve of <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>.</p>" ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Fig. 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 626 "Ancho" => 1341 "Tamanyo" => 58703 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">The curve of <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>.</p>" ] ] 3 => array:8 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at1" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "leyenda" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Description: The data in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a> is regressed with variations in concentration as the <span class="elsevierStyleItalic">X</span> value and % antioxidant activity as the <span class="elsevierStyleItalic">Y</span> value according to the variation in the test method.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Concentration (μg/ml) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Absorbance (<span class="elsevierStyleItalic">A</span>) <span class="elsevierStyleItalic">λ</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>515<span class="elsevierStyleHsp" style=""></span>nm \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Antioxidant activity (%) \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.431 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.46 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">400 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.417 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3.70 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">800 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.390 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">9.93 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1600 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.355 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">18.01 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.271 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">37.41 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Control \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.433 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab2781699.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">DPPH test results table on cocktail honey (Simplo).</p>" ] ] 4 => array:8 [ "identificador" => "tbl0010" "etiqueta" => "Table 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at2" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "leyenda" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Description: The data in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a> is regressed with variations in concentration as the <span class="elsevierStyleItalic">X</span> value and % antioxidant activity as the <span class="elsevierStyleItalic">Y</span> value according to the variation in the test method.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Concentration (μg/ml) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Absorbance (<span class="elsevierStyleItalic">A</span>) <span class="elsevierStyleItalic">λ</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>515<span class="elsevierStyleHsp" style=""></span>nm \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Antioxidant activity (%) \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.426 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.47 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">400 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.422 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1.40 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">800 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.396 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">7.48 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1600 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.356 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">16.82 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.288 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">32.71 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Control \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.428 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab2781700.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">DPPH test results table on cocktail honey (Duplo).</p>" ] ] 5 => array:8 [ "identificador" => "tbl0015" "etiqueta" => "Table 3" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at3" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "leyenda" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall">Description: The data in <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a> is regressed with variations in concentration as the <span class="elsevierStyleItalic">X</span> value and % antioxidant activity as the <span class="elsevierStyleItalic">Y</span> value according to the variation in the test method.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Concentration (μg/ml) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Absorbance (<span class="elsevierStyleItalic">A</span>) <span class="elsevierStyleItalic">λ</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>515<span class="elsevierStyleHsp" style=""></span>nm \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Antioxidant activity (%) \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.416 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">5.88 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">400 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.406 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">8.14 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">800 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.390 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">11.76 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1600 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.350 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">20.81 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.290 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">34.39 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Control \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">0.442 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t"> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab2781701.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">DPPH test results table on cocktail honey (Triplo).</p>" ] ] 6 => array:8 [ "identificador" => "tbl0020" "etiqueta" => "Table 4" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at4" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Testing method \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Line equation \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">Y</span> value \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">X</span> value or IC<span class="elsevierStyleInf">50</span> (μg/ml) \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Simplo \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleItalic">y</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0121<span class="elsevierStyleItalic">x</span><span class="elsevierStyleHsp" style=""></span>−<span class="elsevierStyleHsp" style=""></span>1.0681<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.9969 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">50 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">4220.5041 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">Duplo \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleItalic">y</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0110<span class="elsevierStyleItalic">x</span><span class="elsevierStyleHsp" style=""></span>−<span class="elsevierStyleHsp" style=""></span>1.38107<span class="elsevierStyleItalic">R</span><span class="elsevierStyleSup">2</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.9956 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">50 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">4710.0636 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t ; 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